Search For Gene
Microglia from multiple environments including freshly isolated human microglia (ExVivo, n=17 patients), xMGs (3 iHPC cell lines, n=3-6 mice per line, 13 mice total), iMGLs (n=6 wells), and cultured human microglia (InVitro, n=13 patients) from our lab and Chris Glass’ lab (UCSD; Gosselin et al., (2017) An environment-dependent transcriptional network specifies human microglia identity), were transcriptomically compared. xMG-transplanted mice were aged to 2-6 months depending on cell line.
In vivo LPS
MITRG mice transplanted with xMGs (cell line: AICS-0036) were aged to 2 months then administered 3 injections of LPS (n=5; 2mg/kg; 1 injection/24 hours) or saline (n=3). Mice were euthanized 12 hours after the final injection and RNA was collected for seq.
In vitro LPS
Day 38 iMGLs were plated at 200,000 cells/well on a Matrigel-coated (1mg/well) 24-well-plate containing 2mL of medium. Cells were then treated with either LPS (100ng/mL) or DPBS and incubated for 24 hours at 37°C before lysis for RNA isolation (n=3 wells per treatment group).
If you find this data useful for your work, we would appreciate it if you would cite:
Hasselmann, J., Coburn, M. A., England, W., Figueroa Velez, D. X., Kiani Shabestari, S., Tu, C. H., . . . Blurton-Jones, M. (2019). Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo. Neuron. doi:10.1016/j.neuron.2019.07.002
The datasets included in the plots can be downloaded at:
MBJ iMGL, xMG, and ExVivo
GEO accession number GSE133432
Gosselin InVitro and ExVivo
NCBI dbGaP, accession number phs001373.v1.p1
GEO accession number GSE89189
Please feel free to contact Mathew Blurton-Jones (email@example.com) or Jonathan Hasselmann (firstname.lastname@example.org) with any questions.