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Datasets related to disease and aging models:
1.5 month old wild-type and 5xfAD mice treated until 7 months of age with 1200 ppm PLX5622 (in chow).
Brains microdissected into cortices, hippocampus, and thalamus+striatum.
This dataset accompanies the manuscript "Synthesis of a specific CSF1R inhibitor for sustained microglial depletion reveals crucial roles of microglia in plaque development and transcriptional alterations in Alzheimer's disease mice".
6 week old wild-type and R6/2 mice treated with 275 ppm PLX3397 (in chow) until 11 weeks of age.
From striatal homogenates.
This dataset accompanies the manuscript "Microglia facilitate brain volume loss and extracellular matrix changes in the R6/2 mouse model of Huntington’s disease".
Effects of 2 months treatment of CSF1R+/- mice, a model of ALSP, with the CSF1R inhibitor PLX5622
6 month old wild-type and CSF1R+/- mice treated with 150 ppm PLX5622 (in chow) until 8 months of age.
From cortical homogenates.
This dataset accompanies the manuscript "CSF1R signaling both perturbs and rescues microglial phenotypes and associated presynaptic damage".
Datasets related to microglial/myeloid cell repopulation:
2 month old mice treated with 600 ppm PLX3397 for 7 days (98% elimination of microglia), then drug withdrawn for 7 days to stimulate repopulation, repeated for 2, and 3, cycles.
From whole brains homogenates.
This dataset accompanies the manuscript "A limited capacity for microglial repopulation in the adult brain".
Gene expression in brain extracted microglia and "alternatively" repopulated myeloid cells following systemic LPS administration
8 week old mice treated with PLX3397 (600 ppm in chow) for 14 days, then drug withdrawn to stimulate "alternative" repopulation.
28 days later, mice were administered LPS (IP; 0.33 mg/kg), and then sacrificed 6, and 24 hours later.
Myeloid cells were then extracted via FACS, RNA extracted, and RNA-seq performed.
From brain extracted (FACS) myeloid cells.
This dataset accompanies the manuscript "Complete microglial elimination stimulates full reconstitution of the adult brain with distinct
myeloid cells of meningeal origin, in a wave that spreads from the SVZ".
8 week old mice treated with PLX3397 (600 ppm in chow) for 14 days, then drug withdrawn to stimulate "alternative" repopulation. 5 days later the subcentricular zone was microdissected, RNA extracted, and RNA-seq performe.
From subventricular zone homogenates.
This dataset accompanies the manuscript "Complete microglial elimination stimulates full reconstitution of the adult brain with distinct myeloid cells of meningeal origin, in a wave that spreads from the SVZ" .
Gene Expression changes in Cortex, Hippocampus, and Thalamus with long-term monocyte engraftment
To explore the effects of long-term monocyte engraftment on the brain, we replaced the microglial tissue with monocytes and then waited 6 months.
The brains were extracted and microdissected into cortex, hippocampus, and thalamus/striatum. Bulk tissue RNA was then extracted, and sequencing performed.
This dataset accompanies the manuscript "Selective vulnerability of the hippocampus to long term monocyte engraftment".